Cloning, nucleotide sequence and structural analysis of the Clostridium acetobutylicum dnaJ gene
β Scribed by Susanne Behrens; Franz Narberhaus; Hubert Bahl
- Book ID
- 109319681
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 558 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0378-1097
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## Abstract The DNA fragment of heat shock genes (__hrcAβgrpEβdnaKβdnaJ__) containing complete __hrcAβgrpEβdnaK__ operon and the transcription unit of __dnaJ__ was cloned, sequensed and analyzed from __Bacillus megaterium__ RF5. The sequence of __hrcA, grpE__ and __dnaJ__ were first time reported,
The nucleotide sequence of engD, an endo-beta-1,4-glucanase gene from Clostridium cellulovorans was determined (Genbank Accession No. M37434). The COOH-terminal part of the gene product, EngD, contained a Thr-Thr-Pro repeated sequence followed by a region that has homology to the exoglucanase of Cel