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Cloning, nucleotide sequence and expression of a hemolysin gene of Clostridium septicum

โœ Scribed by Tadashi Imagawa; Yoshitane Dohi; Yasushi Higashi

Book ID
109319906
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
448 KB
Volume
117
Category
Article
ISSN
0378-1097

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The sialidase gene from Clostridium sept
The sialidase gene from Clostridium septicum: cloning, sequencing, expression in Escherichia coli and identification of conserved sequences in sialidases and other proteins
โœ Rothe, Beate ;Rothe, Bernd ;Roggentin, Peter ;Schauer, Roland ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 840 KB

An oligonucleotide mixture corresponding to the codons for conserved and repeated amino acid sequences of bacterial sialidases (Roggentin et al. 1989) was used to clone a 4.3 kb PstI restriction fragment of Clostridium septicum DNA in Escherichia coli. The complete nucleotide sequence of the sialida