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Cloning, sequencing and analysis of dnaK -dnaJ gene cluster of Bacillus megaterium

✍ Scribed by Fangming Bao; Lei Gong; Weilan Shao

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
286 KB
Volume
48
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The DNA fragment of heat shock genes (hrcA‐grpE‐dnaK‐dnaJ) containing complete hrcA‐grpE‐dnaK operon and the transcription unit of dnaJ was cloned, sequensed and analyzed from Bacillus megaterium RF5. The sequence of hrcA, grpE and dnaJ were first time reported, and their coding products exibit 60%, 63% and 81% of identities to the homologs of B. subtilis. A σ^A^‐type promoter of Gram‐positive bacteria (P~A1~) and a terminator were located upstream of the hrcA and downstream of dnaK, and a Controlling inverted repeat of chaperone expression element (CIRCE) was identified between P~A1~ and hrcA. Another σ^A^‐type promoter (P~A2~) and a terminator were found upstream and downstream of dnaJ, indicating B. megaterium has a transcription unit containing a single gene dnaJ. The structure of dnaJ transcription unit is more similar to that of Listeria monocytogenes than other species of Bacillus. A partial protein‐based phylogenetic tree, derived from Gram‐positive bacteria using HrcA sequence, indicated a closer phylogenetic relationship between B. megaterium and Geobacillus species than other two Bacillus species. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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