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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis

✍ Scribed by Reinhard, Linda ;Mueller-Dieckmann, Jochen ;Weiss, Manfred S.


Book ID
117860186
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
485 KB
Volume
68
Category
Article
ISSN
1744-3091

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The recombinant homodimeric hemoglobin from the strictly aerobe gram-negative bacterium Vitreoscilla stercoraria has been expressed in Escherichia coli, purified to homogeneity, and crystallized by vapor diffusion techniques, using ammonium sulfate as precipitant. The crystals belong to the monoclin