Cloning, expression and characterization of four serpin-1 cDNA variants from the spruce budworm, Choristoneura fumiferana
✍ Scribed by Y.-P. Zheng; W.-Y. He; C. Béliveau; A. Nisole; D. Stewart; S.-C. Zheng; D. Doucet; M. Cusson; Q.-L. Feng
- Book ID
- 116345637
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 804 KB
- Volume
- 154
- Category
- Article
- ISSN
- 1096-4959
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## Abstract Two __Choristoneura fumiferana__ glutathione __S__‐transferase cDNAs were cloned from a cDNA library constructed using mRNA from the midgut cell line, CF‐203. These cDNAs (__Cf__GST2, __Cf__GST3) encoded two structurally different proteins with a predicted molecular mass of 21 and 24 kD
Cloning and characterization of a Choristoneura fumiferana ultraspiracle (Cfusp) cDNA are described. First, a PCR fragment and then a cDNA clone (4.4 kb) were isolated from spruce budworm cDNA libraries. Comparison of the deduced amino acid sequence of this cDNA with the sequences in Genbank showed