Cloning and expression of a putative transferrin cDNA of the spruce budworm, Choristoneura fumiferana
β Scribed by D.R. Ampasala; S.-C. Zheng; A. Retnakaran; P.J. Krell; B.M. Arif; Q.-L. Feng
- Book ID
- 116535168
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 450 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0965-1748
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Cloning and characterization of a Choristoneura fumiferana ultraspiracle (Cfusp) cDNA are described. First, a PCR fragment and then a cDNA clone (4.4 kb) were isolated from spruce budworm cDNA libraries. Comparison of the deduced amino acid sequence of this cDNA with the sequences in Genbank showed
## Abstract Two __Choristoneura fumiferana__ glutathione __S__βtransferase cDNAs were cloned from a cDNA library constructed using mRNA from the midgut cell line, CFβ203. These cDNAs (__Cf__GST2, __Cf__GST3) encoded two structurally different proteins with a predicted molecular mass of 21 and 24 kD