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Cloning and functional analysis of molecular chaperone genes fromBacillus stearothermophilusSIC1

✍ Scribed by Katsunori Kohda; Yuji Tsuji; Masahiro Takagi; Tadayuki Imanaka

Publisher: Springer Netherlands
Year: 1996
Tongue: English
Weight: 359 KB
Volume: 18
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00129732

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✦ Synopsis

Genes homologous to groES and groEL, which are recognized as molecular chaperone genes, from Bacillus stearothenT~philus SIC1 were cloned and sequenced.

By addition of GroES, GroEL and ATP in vitro, remaining activity of the alcohol dehydrogenase from Saccharomyces cerevisiae after heat treatment at 50°C for 6 min was improved from 55 % to 90 %.

Furthermore, even though inclusion bodies were formed when asingle chain Fv(sFv) was expressed in E. coil cells, during in vivo coexpressio.n with molecular chaperone, a significant amount of the antibody protein could be recovered from the soluble fraction.

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