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Cloning and expression of an extracellular-agarase gene from Streptomyces coelicolor A3(2) in Streptomyces lividans 66

✍ Scribed by Kendall, Kevin; Cullum, John

Book ID
122092220
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
747 KB
Volume
29
Category
Article
ISSN
0378-1119

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A mutant of Streptomyces coelicolor A 3(2) highly resistant to chloramphenicol was selected. It had amplified some chromosomal DNA fragments to a copy number of 20-50. Some of the amplified fragments were cloned and used as hybridisation probes to investigate the spontaneous chloramphenicol-sensitiv