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Cloning and expression of a thermostable β-1,3-1,4-glucanase fromBacillus amyloliquefaciensATCC 23350

✍ Scribed by Juntao Sun, Hongxin Wang, Wenping Lv, Chaoyang Ma, Zaixiang Lou, Hong Yao, Yixing Dai

Book ID
118302374
Publisher
Springer-Verlag
Year
2011
Tongue
English
Weight
213 KB
Volume
62
Category
Article
ISSN
1590-4261

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Cloning and expression of a β -1,3-gluca
Cloning and expression of a β -1,3-glucanase gene fromBacillus circulansKCTC3004 inEscherichia coli
✍ Dong Seok Lee; Hun-Gil Chang 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 459 KB

A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti