Cloning and expression in Escherichia coli K-12 of the structural gene for outer membrane PhoE protein from Enterobacter cloacae
โ Scribed by Verhoef, Cornelis; van Koppen, Chris; Overduin, Piet; Lugtenberg, Ben; Korteland, Jaap; Tommassen, Jan
- Book ID
- 122824116
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 980 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0378-1119
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๐ SIMILAR VOLUMES
To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat
The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revea