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Cloning of ompF, the structural gene for an outer membrane pore protein of E. coli K12: Physical localization and homology with the phoE gene

โœ Scribed by Tommassen, Jan ;van der Ley, Peter ;van der Ende, Arie ;Bergmans, Hans ;Lugtenberg, Ben


Publisher
Springer
Year
1982
Tongue
English
Weight
687 KB
Volume
185
Category
Article
ISSN
0026-8925

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To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat

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The pldA gene of Escherichia coli K12, which is involved in the synthesis of an outer membrane (OM) phospholipase, has been cloned using a cosmid cloning system. For detection of the cloned gene a newly developed, in vivo phospholipase assay was used. Subsequent cloning of the pldA gene was performe