The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revea
Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12
โ Scribed by Bremer, Erhard ;Beck, Ewald ;Hindennach, Ingrid ;Sonntag, Ingeborg ;Henning, Ulf
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 935 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The regulatory region of the ompA gene from Escherichia coli has been characterized by biochemical and genetic approaches. Two overlapping promoters, P1 and P2, organized in that order with respect to the ompA coding sequence, were identified and it was found that ompA possesses an unusually long le
The pldA gene of Escherichia coli K12, which is involved in the synthesis of an outer membrane (OM) phospholipase, has been cloned using a cosmid cloning system. For detection of the cloned gene a newly developed, in vivo phospholipase assay was used. Subsequent cloning of the pldA gene was performe
To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat