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Chromosomal variation in dividing protoplasts derived from cell suspensions of barley (Hordeum vulgareL.)

✍ Scribed by X.-H. Wang; P. A. Lazzeri; H. Lörz

Book ID
104670363
Publisher
Springer
Year
1992
Tongue
English
Weight
552 KB
Volume
85-85
Category
Article
ISSN
0040-5752

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✦ Synopsis

Numerical and structural chromosome variation was analysed in dividing protoplasts isolated from suspension cells of barley. Five cell lines exhibited distribution patterns in chromosome number with different peaks and ranges. Embryogenic/morphogenic cell lines showed a peak at 2n = 14 (ca. 50%) after 6-7 months in culture, while older non-embryogenic cell lines had peaks at aneuploid or polyploid chromosome numbers. Culture duration had a clear effect on numerical and structural chromosome variation in embryogenic cell lines. With ageing of the cultures chromosome variation accumulated and the proportion of 2n = 14 cells decreased. The effect of protoplast isolation and culture on chromosome variation was examined; more cells with normal chromosome sets (12%) were maintained in protoplast-derived colonies than in source suspension cells (4%) of the same culture age.

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