Biosynthesis of indole-3-acetic acid in protoplasts, chloroplasts and a cytoplasmic fraction from barley (Hordeum vulgareL.)
✍ Scribed by Goran Sandberg; Einar Jensen; Alan Crozier
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 480 KB
- Volume
- 156
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Protoplast preparations from barley
(Hordeum vulgare L.) enzymatically converted [5-3H]tryptophan to [3H]indole-3-acetic acid (IAA). Both a chloroplast and a crude cytoplasmic fraction, isolated from protoplasts that had previously been fed [5-3H]tryptophan, contained [3H]IAA. Chloroplast and cytoplasmic preparations, isolated from protoplasts and thereafter incubated with [5-3H]tryptophan, also synthesized [3H]IAA, although, in both instances the pool size was less than 50% of that detected in the in-vivo feeds. There were no significant differences in the amounts of [3H]IAA that accumulated in protoplast and chloroplast preparations incubated in light and darkness.