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Chromosomal location of the Escherichia coli cytochrome b556 gene, cybA

โœ Scribed by Murakami, Hiroshi ;Kita, Kiyoshi ;Oya, Hiroshi ;Anraku, Yasuhiro

Publisher
Springer
Year
1984
Tongue
English
Weight
492 KB
Volume
196
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

The amounts of cytochrome b556 in the cytoplasmic membranes of several Escherichia coli K12 strains having F-prime factors and a lambda transducing phage were determined. The amount was amplified about two-fold in strains having F100-12 and F152, but not in strains having F100-11, F8 and lambda psu+2glnS+. The strain TK3D11, which lacks the kdp-gltA region (deletion D-01) of the E. coli chromosome, did not synthesize cytochrome b556 at all. From these results, the gene cybA encoding cytochrome b556 was located in the kdp-gltA region. In the cytochrome b556-deficient mutant, a novel b type cytochrome, cytochrome b561 which is a product of the gene cybB, was identified. It seems to function as a physiological electron transferring cytochrome in place of cytochrome b556 in this mutant.

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