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Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12

✍ Scribed by Murakami, Hiroshi ;Kita, Kiyoshi ;Anraku, Yasuhiro

Publisher: Springer
Year: 1984
Tongue: English
Weight: 584 KB
Volume: 198
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00328692

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✦ Synopsis

A 37 kb fragment of DNA from an F-prime factor, F100-12, which showed a gene dosage effect on b-type cytochromes, was cloned with a cosmid vector, pHC79. Gel filtration of cytochromes and product analysis of the hybrid plasmids indicated that this fragment contained cybB, the structural gene for cytochrome b561. A chromosomal DNA fragment carrying the cybB gene was cloned by the plaque hybridization technique with Charon 4A as a vector. The gene was subcloned into pBR322 and was located in a 1.3 kb DNA fragment. It was concluded that the cybB gene is located on the chromosome of Escherichia coli K12.

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