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Chromatography of renin substrate on concanavalin A-agarose

✍ Scribed by Morton P. Printz; Timothy J. Gregory; John A. Lewicki; Jana M. Printz

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
479 KB
Volume
73
Category
Article
ISSN
0003-2697

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✦ Synopsis

Renin substrate, the serum prohormone of angiotensin, is a glycoprotein which is shown to bind to concanavalin A. This interaction permits the use of lectin affinity chromatography in overcoming a major problem in the protein's purification, namely the separation of renin substrate from albumin and similar serum proteins. Low concentrations of either a-methyl mannoside or o-glucose displace renin substrate from the affinity column with no significant loss of pharmacological activity.

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