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Affinity chromatography of soybean β-amylase on phenylboronate agarose

✍ Scribed by Luna, Beatriz ;Brena, Beatriz ;Diaz, Teresita ;Batista-Viera, Francisco


Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
262 KB
Volume
15
Category
Article
ISSN
0935-6304

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UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characte