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Affinity chromatography of endoglucanase of Trichoderma viride by concanavalin A–agarose

✍ Scribed by Cheng-Shung Gong; Li Fu Chen; George T. Tsao

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
186 KB
Volume
21
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

Endoglucanase (C~x~ cellulase) and cellobiase are often cross‐contaminated in separation procedures by ion‐exchange chromatography such as DEAE‐cellulose. By using concanavalin A (Con A)–agarose chromatography, C~x~ cellulase and cellobiase from Trichoderma viride can be separated. C~x~ cellulase showed affinity toward Con A, indicating a glycoprotein containing α‐D‐mannopyransyl and α‐D‐glucopyranosyl end groups or internal 2‐O‐D‐mannopyranosyl residues in sugar moieties. This method provides a way to estimate the quantities of C~x~ enzyme produced by T. viride and possibly by other organisms.

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