Chromatography of Nucleic Acids on Hydroxyapatite
β Scribed by BERNARDI, GIORGIO
- Book ID
- 109644037
- Publisher
- Nature Publishing Group
- Year
- 1965
- Tongue
- English
- Weight
- 526 KB
- Volume
- 206
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/206779a0
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π SIMILAR VOLUMES
Cyclodextrins are cyclic a-1,4-oligoglucosides having six or more anhydroglucose residues per molecule. As a result of their cyclic nature, these carbohydrate molecules possess a cavity within which various small molecules have been shown to bind by a process which has come to be called inclusion co
## Abstract | I. | Introduction | 311 | | | A. Nucleic Acid Structures and Properties | 311 | | | B. Principles of LC of Nucleic Acids | 312 | | | ββ1. ReversedβPhase HPLC | 312 | | | ββ2. IonβPair ReversedβPhase HPLC | 314 | | | ββ3. Affinity Chromatography | 314 | | | C. Principles of ESI M
Preparations of rabbit rnyosin have been subjected to chromatographic fractionation on columns of hydroxyapatite at pH 6.8 with potassium phosphate buffers containing RCl. The best resolution is obtained with a stepwise elution procedure, utilizing phosphate concentrations of o.IG, 0.23, and 0~4 M,