Chromatography of nucleic acid components on cyclodextrin gels

✍ Scribed by Jerald L. Hoffman

• Publisher: Elsevier Science
• Year: 1970
• Tongue: English
• Weight: 473 KB
• Volume: 33
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(70)90288-5

✦ Synopsis

Cyclodextrins are cyclic a-1,4-oligoglucosides having six or more anhydroglucose residues per molecule. As a result of their cyclic nature, these carbohydrate molecules possess a cavity within which various small molecules have been shown to bind by a process which has come to be called inclusion complexing. Two recent reviews have dealt with the formation of cyclodextrin inclusion complexes (1, 2)) and illustrate the large variety of molecules which can interact in this manner.

Nucleic acid components, particularly those containing adenine bases, have been shown to form inclusion complexes with cycloheptaamylose but not cyclohexaamylose

(3). The strength of the interactions of the following compounds with cycloheptaamylose as indicated by the magnitude of their ultraviolet spectral change on complexing was found to be:l 3'-AMP > 5'-AMP > ATP > 2',3'-cyclic AMP > ADP > 2'-AMP = adenosine > adenine 'Abbreviations. A, C, G, U, T indicate adenosine, cytidine, guanosine, uridine, thpmidine, respectively; prefix "d" denotes the presence of 2'-deoxyribose (not required for thymidine).