Methyl ethers (6a and 6b) of N-trifluoroacetyl-and N-(m-trifluoromethyl) phenylsulfonyl-6-nitro-tyrosine t-butyl ester were readily prepared by modified Mitsunobu reaction (DPPE, DIAD, MeOH). Williamson (MeI, K 2 CO 3 or Li 2 CO 3 or NaOH under phase transfer) and classical Mitsunobu conditions (PPh
✦ LIBER ✦
Chromatographic resolution of molecular species of phosphatidylethanolamine as n-acyl-o-methyl derivatives
✍ Scribed by R. Sundler; B. Åkesson
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 613 KB
- Volume
- 80
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
Phosphatidylethanolamines
were converted into the N-acetyl-O-methyl or N-benzoyl-O-methyl derivatives by treatment with acetic anhydride/benzoyl chloride and diazomethane, Methods for the separation of these derivatives by argentation thin-layer chromatography and reversed-phase partition thin-layer chromatography are described. The procedure is especially advantageous for the analysis of phosphatidylethanolamines that are radioactively labelled in the ethanolamine moiety.
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