Preparation of cytochrome c peroxidase f
โ
C.E. Nelson; E.V. Sitzman; C.H. Kang; E. Margoliash
๐
Article
๐
1977
๐
Elsevier Science
๐
English
โ 580 KB
A simple and readily reproducible procedure is presented for the preparation and purification of cytochrome c peroxidase from baker's yeast. Following autolysis of the yeast and extraction, the enzyme is collected on DEAE-cellulose at moderately high ionic strength, eluted, concentrated, and subject