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Circular dichroism studies on cytochrome c peroxidase from baker's yeast (Saccharomyces cerevisiae)

✍ Scribed by Gunnel Sievers


Book ID
118309076
Publisher
Elsevier Science
Year
1978
Weight
785 KB
Volume
536
Category
Article
ISSN
0005-2795

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A simple and readily reproducible procedure is presented for the preparation and purification of cytochrome c peroxidase from baker's yeast. Following autolysis of the yeast and extraction, the enzyme is collected on DEAE-cellulose at moderately high ionic strength, eluted, concentrated, and subject

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## Abstract Circular dichroism studies on synthetic peptides related to the C‐terminal region of yeast iso‐1‐cytochrome c were carried out and compared with conformational studies on horse cytochrome c fragments. Evidence is presented for a weaker predisposition for ordered structure in the former