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Preparation of cytochrome c peroxidase from baker's yeast

✍ Scribed by C.E. Nelson; E.V. Sitzman; C.H. Kang; E. Margoliash

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
580 KB
Volume
83
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple and readily reproducible procedure is presented for the preparation and purification of cytochrome c peroxidase from baker's yeast. Following autolysis of the yeast and extraction, the enzyme is collected on DEAE-cellulose at moderately high ionic strength, eluted, concentrated, and subjected to gel filtration in 0.1 M sodium acetate buffer, pH 5.0. The properties of the crude preparation make gel filtration in this buffer suitable for near-final purification of the heme protein. The enzyme is then easily crystallized by dialysis.

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