Covalent complexes of yeast cytochrome c peroxidase and yeast cytochrome c
โ Scribed by Stamatia Chroni; Bih-Show Lou; SusanJ. Moench; JamesE. Erman; JamesD. Satterlee
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 82 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0162-0134
No coin nor oath required. For personal study only.
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A simple and readily reproducible procedure is presented for the preparation and purification of cytochrome c peroxidase from baker's yeast. Following autolysis of the yeast and extraction, the enzyme is collected on DEAE-cellulose at moderately high ionic strength, eluted, concentrated, and subject
The addition of exogenous ligands to the ferric and ferrous states of yeast cytochrome c peroxidase (CCP) is investigated with magnetic circular dichroism (MCD) at 4ยฐC to determine the effect the protein environment may exercise on spectral properties. The MCD spectrum of each derivative is directly