Chromatographic aspects in high throughput liquid chromatography/mass spectrometry

✍ Scribed by Luisa Pereira; Paul Ross; Mark Woodruff

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 63 KB
• Volume: 14
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(20000315)14:5<357::aid-rcm889>3.0.co;2-b

✦ Synopsis

Important parameters to consider when developing a liquid chromatography/mass spectrometry (LC/MS) method are buffer type and concentration, and column geometry. In the work presented here the choice of buffer for the analysis of basic compounds using a polar embedded phase (HyPURITYtrade mark ADVANCE) is illustrated for the analysis of tricyclic antidepressants. Method transfer from a 4.6 mm i.d. column to a 2.1 mm i.d. column is demonstrated for the analysis of triazines and anabolic steroids and their metabolites, with no change in selectivity and with added speed of analysis. Analysis of eight beta-blockers is achieved in 65 seconds by using a short 30 x 4.6 mm C18 column.