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Chemokines derived from soluble fusion proteins expressed in Escherichia coli are biologically active

โœ Scribed by Giovanni Magistrelli; Franck Gueneau; Machadiya Muslmani; Ulla Ravn; Marie Kosco-Vilbois; Nicolas Fischer

Book ID
116290857
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
540 KB
Volume
334
Category
Article
ISSN
0006-291X

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โœ Gregory D. Davis; Claude Elisee; Denton M. Newham; Roger G. Harrison ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 214 KB

Three native E. coli proteins-NusA, GrpE, and bacterioferritin (BFR)-were studied in fusion proteins expressed in E. coli for their ability to confer solubility on a target insoluble protein at the C-terminus of the fusion protein. These three proteins were chosen based on their favorable cytoplasmi