Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners
โ Scribed by Seonghun Kim; Sun Bok Lee
- Book ID
- 116828856
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 286 KB
- Volume
- 62
- Category
- Article
- ISSN
- 1046-5928
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,
Three native E. coli proteins-NusA, GrpE, and bacterioferritin (BFR)-were studied in fusion proteins expressed in E. coli for their ability to confer solubility on a target insoluble protein at the C-terminus of the fusion protein. These three proteins were chosen based on their favorable cytoplasmi