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ChemInform Abstract: β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase.

✍ Scribed by Oyekanmi Nashiru; David L. Zechel; Dominik Stoll; Taraneh Mohammadzadeh; R. Antony J. Warren; Stephen G. Withers

Publisher
John Wiley and Sons
Year
2001
Weight
26 KB
Volume
32
Category
Article
ISSN
0931-7597

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Engineering enzymes: The glutamic acid nucleophile of a retaining β-mannosidase has been replaced with a serine residue to form a β-mannosynthase. When the new enzyme is provided with an α-mannosyl fluoride donor and an appropriate acceptor, β-mannoside linkages are synthesized. Remarkably, α-mannos

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