ChemInform Abstract: Efficient and Enantioselective Catalysis by the Semisynthetic Peroxidase Seleno-Subtilisin.
β Scribed by D. HAERING; M. HERDERICH; E. SCHUELER; B. WITHOPF; P. SCHREIER
- Publisher
- John Wiley and Sons
- Year
- 2010
- Weight
- 30 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0931-7597
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β¦ Synopsis
Efficient and Enantioselective Catalysis by the Semisynthetic Peroxidase Seleno-Subtilisin.
-It is found that alkyl aryl hydroperoxides can be resolved efficiently by seleno-subtilisin. Seleno-subtilisin shows a catalytic efficiency comparable to native enzymes and an enantioselectivity assessable from known subtilisin substrates.
-(HAERING, D.; HERDERICH, M.;
π SIMILAR VOLUMES
The linkage of lysine residues on the surfaces of subtilisin crystals (NH -Enz; see Scheme) with glutardialdehyde affords an immobilized biocatalyst of high stability and purity. The replacement of the serine OH group in the active site (Enz-OH) by SeO H leads to new activity as a peroxidase. Thus f
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