Chemiluminescent immunoassay (CLIA) for urinary albumin

✍ Scribed by Bagazgoitia, F. Javier ;Garcia, J. Luis ;Ibarra, J. M. ;Patiño, R. ;Diéquez, Carlos ;Weeks, Ian ;Woodhead, J. Stuart

• Publisher: John Wiley and Sons
• Year: 1989
• Weight: 407 KB
• Volume: 3
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170030403

✦ Synopsis

A simple chemiluminescent immunoassay (CLIA) for urinary albumin has been developed based on the use of a chemiluminescent acridinium ester-labelled human albumin and a commercially available antiserum. It includes t w o incubation steps and a second polyethylene glycol-assisted antibody separation. The sensitivity of detection is 0.016 mg/l, the assay working range is 0.1-5mg/l, and the inter-assay CVs are S15%. Using 10-and 50-fold sample dilutions in assay buffer, a wide working range (1-250mg/l) is obtained covering normal and pathological conditions. Timed overnight urine samples (bed rest conditions) were collected on three consecutive days for each patient. Albumin excretion rate (AER) was 4.7 f 2.7 pglmin (x & SD), range 1-15.9 pg/min in 36 healthy subjects (176". 199, ages 4 4 6 years), with day-to-day variations of 28.5 f 20% (k f SD), range 3.3-76.1 %. The use of an acridinium ester as a chemiluminescent (CL) label overcomes the disadvantages of short shelf-life and health and safety hazards associated with radioisotopes. Results compare favourably with those obtained using a commercially available RIA kit.