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Enhanced chemiluminescent sandwich enzyme immunoassay for hen egg lysozyme

✍ Scribed by Rauch, Pavel ;Poplstein, Martin ;Hochel, Igor ;Fukal, Ladislav ;Ferri, Elida ;Abagnato, Carlo Alberto ;Girotti, Stefano ;Roda, Aldo

Publisher: John Wiley and Sons
Year: 1995
Weight: 408 KB
Volume: 10
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170100106

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✦ Synopsis

A sensitive chemiluminescent sandwich-type enzyme immunoassay for hen egg lysozyme was developed. The assay was performed on polystyrene microtitre plates using immobilized specific polyclonal rabbit antibody against lysozyme, a peroxidase conjugate and the H202/luminol-enhanced chemiluminescence detection reagent. The chemiluminescent signal was detected using either a microplate luminometer, or photographic film in a camera luminometer. The detection limit for lysozyme was 0.3ng/mL, and this was three times lower than that obtained using a colorimetric method w i t h H202 and o-phenylendiamine as substrates. Recovery o f the assay was 97-112% and the relative standard deviation ranged from 3.6% t o 10.3%. The immunoassay overcame interference from the food sample matrix when lysozyme, used as a bacteriostatic agent, was measured.

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