Chemiluminescence sandwich enzyme immunoassay for determination of human granulocyte colony stimulating factor (G-CSF)

✍ Scribed by Aoyagi, Shigeo ;Arasawa, Kazue ;Matsuyuki, Akira ;Kamatchi, Shin-ichi ;Fukushima, Masafumi ;Ohsawa, Nakaaki

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 432 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100607

✦ Synopsis

A chemiluminescence sandwich enzyme immunoassay, using a glucose oxidase (GO) label, was developed for detecting attomole amounts o f human granulocyte colony stimulating factor (G-CSF). Purified goat F(ab')* immobilized on a bead and purified goat Fab' labelled with GO were selected in combination w i t h a chemiluminescent detection system comprising luminol and ferricyanide. The detection limits for G-CSF were 4amol/assay (1 pg/mL) in buffer solution and lOamol/assay (2.5 pg/mL) in human serum. Coefficients o f variation within assay and between assay ranged from 5.5% t o 7.8% and from 3.4% t o 16.0%. respectively. The G-CSF content of serum from normal healthy individuals was measurable using this method. G-CSF in 24 normal human sera showed a mean value o f 19.3pg/mL and ranged from 3.6 t o 83.0 pg/mL.