Granulocytic colony-stimulating factor (G-CSF) does not induce differentiation of WEHI3B(D+) cells but is required for the survival of the mature progeny
✍ Scribed by Ralph M. Böhmer; Antony W. Burgess
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- French
- Weight
- 638 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
It has been reported previously that cells of the murine myelomonocytic leukaemia cell line WEH13B(D+) can be induced to differentiate into mature monocytes by incubation with post-endotoxin serum (ES), granulocyte-colony-stimulating factor (G-CSF) or actinomycin D (AMD). We have investigated the kinetics of the differentiation process in suspension cultures, using growth curves and morphological markers to analyze proliferation and differentiation. At low cell densities (<I05/ml) neither ES nor G-CSF changed the rate of WEH13B(D+) proliferation; nor did these reagents induce the differentiation of WEH13B(D+). In contrast, AMD caused growth arrest and initiated cell maturation, which was followed by cell death. The time course of maturation depended on AMD concentration. The addition of either ES or G-CSF at the time of AMD exposure or at times up to 20 hr after the beginning of AMD exposure resulted in a delay of cell death for up to 3 days and in an accumulation of mature monocytes. The kinetics of AMD-induced maturation were not affected by the time of ES or G-CSF addition. We conclude that neither ES nor G-CSF induce the differentiation of WEH13B(D+) cells directly, but that their presence permits the survival of mature cells produced from WEH13B(D+) as a result of a shortrange autocrine-induced differentiation.