Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl-ethanolamine in plasma
✍ Scribed by Gay, B. ;Towbin, H. ;Schnell, C. ;Einsle, K. ;Graf, P. ;Gygax, D.
- Publisher
- John Wiley and Sons
- Year
- 1991
- Weight
- 449 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
Abstract
A competitive chemiluminescent immunoassay for quantitation of muramyl tripeptide phosphatidyl‐ethanolamine (MTP‐PE) in plasma has been developed. The assay is based on the use of an acridinium ester‐labelled analogue of muramyl tripeptide and a rabbit antiserum. It includes an overnight incubation and a separation with a second antibody covalently coupled to paramagnetic particles. The sensitivity of detection is 0.012 nmol/l, the assay working range is 0.1‐5 nmol/l, and the inter‐assay CVs are ⩽ 10%. Using up to 6000‐fold sample dilutions, a wide working range (0.1‐30 000 nmol/l) is obtained. Rat plasma samples were collected during and one day after intravenous infusion of MTP‐PE. Following infusion, the concentrations in plasma declined multiphasically. Half‐life time was 0.37 h ± 0.03 (mean ± SD, alpha phase) and 1.76 h ± 0.08 (mean ± SD, beta phase), clearance and volume of distribution were 0.09 ± 0.02 l/h × kg (mean ± SD) and 0.06 ± 0.01 l/kg (mean ± SD) respectively. The use of an acridinium ester as a chemiluminescent (CL) label overcomes the problems associated with reagents of limited shelf‐life.