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Chemiluminescent determination of tryptophan in a flow injection system

✍ Scribed by Abdulrahman A. Alwarthan

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
411 KB
Volume
317
Category
Article
ISSN
0003-2670

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✦ Synopsis

A method is described for the determination of tryptophan in which it reacts with cerium(IV) in 0.15 M sulphuric acid. The method is rapid and precise and solutions can be analysed at a rate of 211 h-i with a relative standard deviation of ca. 0.45% at 12.5 pg ml-' and a limit of detection of 0.1 pg ml -I. The effect of some common amino acids and monosaccharides which may be present along with tryptophan in tissue or protein hydrolysates has been investigated.

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