To assess the effect of sulphite on the oxidative metabolism of human neutrophils, chemiluminescence (CL) measurements were performed using lucigenin and luminol as chemiluminigenic probes. Lucigenin-dependent CL was used for measuring superoxide anion (0;) production, and luminol-dependent CL was u
Chemiluminescence of lucigenin is dependent on experimental conditions
โ Scribed by Pavel Hyrsl; Antonin Lojek; Milan Ciz; Lukas Kubala
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 74 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.755
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Alveolar macrophages (AM) were studied for their capability to release mediators involved in modulation of neutrophil (PMN) functions. Initial responses were induced by sulphite. Supernatants obtained from canine, human and rat AM pre-treated with sulphite in concentrations of 0.1ยฑ2 mmol/L enhanced
The chemiluminescence of luminol and lucigenin is often used to detect the production of reactive oxygen derivatives by phagocytic cells. Also, several quenchers and enzyme inhibitors are used to determine which oxygen derivatives are responsible for the observed effects. In the present work we have
## Abstract Three types of flow reactors with different lengths (12โ126โ mm) and widths (0.4โ2.0โ mm) of channel were made on the silicon chip by microfabrication techniques for the chemiluminescent (CL) detection of epinephrine (EP) with lucigenin (Luc). The volume of each CL reactor was about 10โ ยตL