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The effect of quenchers on the chemiluminescence of luminol and lucigenin

✍ Scribed by Bottu, G.

Publisher
John Wiley and Sons
Year
1989
Weight
520 KB
Volume
3
Category
Article
ISSN
0884-3996

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✦ Synopsis

The chemiluminescence of luminol and lucigenin is often used to detect the production of reactive oxygen derivatives by phagocytic cells. Also, several quenchers and enzyme inhibitors are used to determine which oxygen derivatives are responsible for the observed effects. In the present work we have assessed the reliability of dimethylthiourea and cysteamine (OHquenchers), desferrioxamine (iron chelator) and diethyldithiocarbamate (superoxide dismutase inhibitor). They all react with CIO-and are also stiong inhibitors of the luminescence of luminol catalysed by horseradish peroxidase (HRP); cysteamine and diethyldithiocarbamate also react with HZ02. NaN3 is an inhibitor of myeloperoxidase and a quencher of singlet 0,. but we found that under certain conditions it can amplify the the luminescence of luminol triggered by CIOor Fenton's reagent. A complex of copper and penicillamine that had been proposed as an 0 z 7 quencher, quenches all luminescent reactions studied. On the other hand, we were able t o confirm the relative specificity of other quenchers: taurine for CIO-, benzoate for OH. and mannitol for both OH. and 'crypto-OH".

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