Chemiluminescence determination of guanine and its nucleosides and nucleotides using phenylglyoxal

✍ Scribed by Masaaki Kai; Yosuke Ohkura; Sayuri Yonekura; Masatake Iwasaki

• Book ID: 102981274
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 597 KB
• Volume: 287
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)85103-4

✦ Synopsis

A novel chemiluminescence method is described for the determination of guanine and its nucleosides and nucleotides. The method is based on the fluorescence reaction of the substances with phenylglyoxal in a phosphate buffer (pH 6.0) at 37°C for 20 min, followed by a chemiluminescence reaction with N,N-dimethylformamide in a weakly alkaline medium. The established conditions of the chemiluminescence reaction do not permit any development of chemi1uminescence from other nucleic acid bases such as adenine, cytosine, uracil and thymine, and their nucleo&)ides. This method is quite selective and approximately 2O-fold more sensitive than the fluorimetric one; the detection limits for guanine nucleosft)ides are 4-19 pmol ml-' in the reaction mixture.