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Alkoxyphenylglyoxals as fluorogenic reagents selective for guanine and its nucleosides and nucleotides in liquid chromatography

✍ Scribed by Yoshihito Ohba; Masaaki Kai; Hitoshi Nohta; Yosuke Ohkura

Book ID: 102982266
Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 527 KB
Volume: 287
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)e0596-y

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✦ Synopsis

Glyoxal analogues which have a phenyl moiety substituted with electron-donating methoxyl group(s) or a methylenedioxyl group, react selectively with guanine and its nucleosides and nucleotides in phosphate buffer (pH 7.0) at 37°C for 5-7 min to give the corresponding fluorescent derivatives. The derivatives can be separated by reversed-phase liquid chromatography. 3,4-Dimethoxyphenylglyoxal is most sensitive; the detection limits (S/N = 3) for guanine and its nucleosides and nucleotides vary in the range 40-400 fmol per injection volume depending on the guanine-related compounds.

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