Chemiluminescence arising from the oxidation of bilirubin in aqueous media

✍ Scribed by L.P. Palilis; A.C. Calokerinos; N. Grekas

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 711 KB
• Volume: 333
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(96)00279-6

✦ Synopsis

Novel chemiluminogenic

redox reactions of bilirubin in aqueous media are reported. N-bromosuccinimide and sodium hypochlorite were the oxidants which generated the most intense radiation and the corresponding reactions were monitored using a batch chemiluminometer.

Consequently, the reaction of bilirubin with N-bromosuccinimide was further investigated using a flow injection analyser and the reaction, with hypochlorite, was further examined by using a continuous flow analyser in order to automate the procedure. The results indicate that both procedures are very sensitive (batch procedure with NaClO: linear range 0.2-20 pg ml-'; 317 detection limit 50 ng ml-') since bilirubin at concentrations less than 1 ng ml-' can be easily and reproducibly measured. Albumin interferes seriously by enhancing the emission.