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Chemical Mechanism and Specificity of the C5-Mannuronan Epimerase Reaction †

✍ Scribed by Jerga, Agoston; Stanley, Matthew D.; Tipton, Peter A.


Book ID
120392521
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
121 KB
Volume
45
Category
Article
ISSN
0006-2960

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A mannuronan C-5-epimerase from the soil bacterium Azotobacter vinelundii has been highly purified and characterised. The enzyme is a single polypeptide chain with a mobility in gradient and SDS electrophoresis corresponding to a mol. wt. of 122,000; it has a p1 of 4, highest activity at pH 7.0, and