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Mode of action of recombinant Azotobacter vinelandii mannuronan C-5 epimerases AlgE2 and AlgE4

✍ Scribed by Martin Hartmann; Olav B. Holm; Gunn A. B. Johansen; Gudmund Skjåk-Bræk; Bjørn T. Stokke


Book ID
101716521
Publisher
Wiley (John Wiley & Sons)
Year
2002
Tongue
English
Weight
196 KB
Volume
63
Category
Article
ISSN
0006-3525

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A mannuronan C-5-epimerase from the soil bacterium Azotobacter vinelundii has been highly purified and characterised. The enzyme is a single polypeptide chain with a mobility in gradient and SDS electrophoresis corresponding to a mol. wt. of 122,000; it has a p1 of 4, highest activity at pH 7.0, and