Action pattern of mannuronan C-5-epimerase: generation of block-copolymeric structures in alginates by a multiple-attack mechanism

NMR spectroscopy studies of the action p

NMR spectroscopy studies of the action pattern of tomato pectinesterase: generation of block structure in pectin by a multiple-attack mechanism

✍ Hans Grasdalen; Anne Kath Andersen; Bjørn Larsen 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 492 KB

The enzymatic de-esterification of highly esterified poly(D-galacturonate) (degree of esterification, d.e. = 0.92, dpn --15) was monitored by 1H NMR spectroscopy. The enzymatic reaction resulted in a high content of homogeneous triads (GGG and EEE) demonstrating the production of a sequential struct