Chemical Cleavage Sequencing of DNA Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
β Scribed by Isola, Narayana R.; Allman, Steve L.; Golovlov, Valerie V.; Chen, Chung-Hsuan
- Book ID
- 127391318
- Publisher
- American Chemical Society
- Year
- 1999
- Tongue
- English
- Weight
- 74 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0003-2700
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Chemical degradation methods combined with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and post-source decay (PSD)-MALDI reflex TOF mass spectrometry (MS) were used to determine the sequence of a peptide branched on to a known peptide backbone. This study was applied to a
Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing