Using the thermoconditional yeast mutant rev2ts that controls an apparently site-specific step of mutagenic DNA repair it was possible to measure the time course of REV2 dependent UV-induced reversion of the ochre allele his5-2 and recovery of survival for UV-treated stationary phase cells: due to t
Checkpoint-Dependent Activation of Mutagenic Repair in Saccharomyces cerevisiae pol3-01 Mutants
β Scribed by Abhijit Datta; James L. Schmeits; Neelam S. Amin; Patrick J. Lau; Kyungjae Myung; Richard D. Kolodner
- Book ID
- 117735949
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 185 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1097-2765
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The time course of REV2 dependent recovery from prelethal UV damage and UV-induced locus-specific reversion of the his5-2 allele was determined in temperature-shift experiments by use of a thermoconditional allele of the rev2 gene (rad5-8, rev2ts). In UV-irradiated, exponentially growing rev2ts cell
The repair of UV-irradiated DNA of plasmid pBB29 was studied in an incision-defective rad3-2 strain of Saccharomyces cerevisiae and in a uvrA6 strain of Escherichia coli by the measurement of cell transformation. Plasmid pBB29 used in these experiments contained as markers the DNA of nuclear yeast g