Characterization of thermosensitive mutants of Physarum polycephalum
β Scribed by Del Castillo, L. ;Oustrin, M. L. ;Wright, M.
- Book ID
- 104692953
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 860 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0026-8925
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A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the
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