๐”– Bobbio Scriptorium
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Revertants of selfing(gad) mutants in Physarum polycephalum

โœ Scribed by Anderson, Roger W. ;Holt, Charles E.

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
867 KB
Volume
2
Category
Article
ISSN
0192-253X

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โœฆ Synopsis

Abstract

The conversion of the uninucleate amoebal form of Physarum polycephalum to the multiโ€nucleate plasmodial form is under the control of a genetic region which contains matA (or mt), a determinant of mating specificity. The region is the site of most gad mutations, which give amoebae the ability to produce plasmodia in clones without mating (ie, to self). In the present study, nonselfing revertants were isolated from two matA2โ€derived gad mutants and two matA3โ€derived gad mutants. Some revertants were found to have regained exactly, or nearly, the same phenotype as the original matA2 or matA3 strain. Others expressed new mating types, having gained the ability to mate with strains of the parental matA type. The results are compatible with a model in which new mating types arise from forward mutations (gad) and back mutations (npf or no plasmodium formation) occurring successively in a single gene, matA.

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