Characterization of the genetic system of the xylose-fermenting yeastPichia stipitis
β Scribed by Tsega Melake; Volkmar Passoth; Ulrich Klinner
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 567 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0343-8651
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Two xylanolytic enzymes, xylanase and flxylosidase from the yeast Pichia stipitis were purified to homogeneity and characterized. Both enzymes are secreted into the culture medium upon growth on xylan. The xylanase is a glycoprotein with an approximate molecular mass of 43 kDa. The N-linked carbohyd
The xylose-utilizing yeast, Pichia stipitis, has a complex respiratory system that contains cytochrome and non-cytochrome alternative electron transport chains in its mitochondria. To gain primary insights into the alternative respiratory pathway, a cytochrome c gene (PsCYC1, Accession No. AF030426)
Two Pichia stipitis ADH genes (PsADH1 and PsADH2) were isolated by complementation of a Saccharomyces cerevisiae Adh -mutant. The genes enabled the transformants to grow in the presence of antimycin A on glucose, to use ethanol as sole carbon source and made them sensitive to allylalcohol. The sequ